Cell Migration Assay | Oris Detection Zone Technology


The Oris™ Cell Migration Assay platform uses a 96-well plate with “stopper” barriers that create a central cell-free Detection Zone for cell migration experiments.  Removing the stoppers allows the cells to migrate into the Detection Zone at the center of each well.

Schematic - procedure for conducting a cell migration assay.


Oris™ Cell Migration Assay kits provide consistent Detection Zones to give you superior reproducibility, accuracy and precision in your experiments.  The Oris™ Cell Migration Assay is the best option for publication-ready, high-quality data with fewer samples.

Data on cell migration obtained with Oris Assay vs Scratch assay


The Oris™ Cell Migration Assay kits can be used with any adherent cell line, and the wells of the assay are coated with an extracellular matrix (Tissue Culture, Fibronectin or Collagen I) to support cell migration of the most common cell types.  Additionally, this assay is compatible with plate readers and high-content analyzers, so you can rapidly quantify cell migration at any time during the duration of your experiment.

Review our application notes to learn more about instruments compatible with the Oris Cell Migration Assay



Use the Oris™ Cell Migration Assay kits to identify cell migration inhibitors and cell migration promoters.  For researchers in academia and pharmaceutical companies, the Oris™ Cell Migration Assay is a powerful tool to advance studies in drug discovery, wound healing, or cancer research.

Learn how to perform a cell migration assay:  Discover our protocol for cell migration assays and our posters on cell migration assays.

Single Plate Kits:

SKUProduct Description
CMA1.101Oris Cell Migration Assay | Tissue Culture-Treated | 96-wellsBUY NOW
CMACC1.101Oris Cell Migration Assay | Collagen Coated | 96-wellsBUY NOW
CMAFN1.101Oris Cell Migration Assay | Fibronectin Coated | 96-wellsBUY NOW


Five Plate Kits:

SKUProduct Description
CMA5.101Oris Cell Migration Assay | Tissue Culture-Treated | 5x96-wellsBUY NOW
CMACC5.101Oris Cell Migration Assay | Collagen Coated | 5x96-wellsBUY NOW
CMAFN5.101Oris Cell Migration Assay | Fibronectin Coated | 5x96-wellsBUY NOW



Whereas most Oris™ Cell Migration Assay products have the stoppers in place already, stoppers are packaged separately in the FLEX and Universal kits:

  • The Oris™ FLEX kit is provided with four empty plates and four packs of 24 stoppers.
    • Use the FLEX kit if you want to analyze fewer than 96 samples per experiment but still want to take advantage of the cost savings and accelerated assay time that small wells offer.
  • The Oris™ Universal Cell Migration Assembly Kits are provided with one empty plate per pack of 96 stoppers.  Available as a 1-pack or 5-pack.
    • Use the Universal kit when you want to apply your own coating to the plate.

Learn how to setup the Universal Assembly kit.  Check out our video HERE.

SKUProduct DescriptionPackage Size
Oris™ 96-well plates + stoppers (not inserted)
Wells are Tissue Culture Treated
4 plates
+96 stoppers
CMAU101Universal kit
Oris™ 96-well plate + stoppers (not inserted)
Wells are Tissue Culture Treated
1 plate
+96 stoppers
CMAU505Universal kit
Oris™ 96-well plates + stoppers (not inserted)
Wells are Tissue Culture Treated
5 plates
+480 stoppers


Examples of Studies that used the Oris™ Cell Migration Assay:

  • Y. Lee et al. “Downregulation of PRMT1 promotes the senescence and migration of non-MYCN amplified neuroblastoma SK-N-SH cells” Sci Rep 9, 177; 2019. LINK
  • A. Kanda et al. “TGF-β-SNAIL axis induces Muller glial-mesenchymal transition in the pathogenesis of idiopathic epiretinal membrane” Sci Rep 9, 673; 2019. LINK
  • M. Gnanamony et al. “Chronic radiation exposure of neuroblastoma cells reduces nMYC copy number” Oncology Lett. 14(3); 2017. LINK
  • H. Marei et al. “Differential Rac1 signalling by guanine nucleotide exchange factors implicates FLII in regulating Rac1-driven cell migration” Nature Comm. 7; 10664; 2016. LINK
  • S. Ravi et al. “Incorporation of fibronectin to enhance cytocompatibility in multilayer elastin-like protein scaffolds for tissue engineering” J. Biomed Mater. Res. A 101(7); 2013. LINK
  • W. Gough et al. A Quantitative, Facile, and High-Throughput Image-Based Cell Migration Method Is a Robust Alternative to Scratch Assay” J. Biomol. Screen. 16(2); pp.155-163; 2011. LINK

Oris technology is protected by US patents 7,842,499, 8,268,614 and 8,512,974 and pending applications.
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