Cell Migration & Invasion Assays

Cell Migration & Invasion Assays by Platypus Technologies use exclusion zone technology to ensure consistent cell-free regions for each experiment.  The Oris™ and Oris™ Pro Cell Migration and Invasion assays enable accurate and reproducible quantification of cell movement.

Cell Migration Assays

Advantages:

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  • Industry-standard 96- or 384-well formats
  • Real-time monitoring
  • Quantification with plate readers, microscopes or high-content imagers
  • No artificial membranes
  • Use Oris™ for maximal assay flexibility
  • Use Oris™ Pro for full automation with high Z factors

Oris™ Cell Migration Assay

Assays use physical “stopper” barriers to create a central cell-free detection zone in the center of each well of a 96-well plate.

Oris™ Pro Cell Migration Assay

Assays use soluble gel spot to create a central cell-free detection zone in each well of 96- or 384-well plates.

Characteristics

Oris™ assays use physical “stopper” barriers to create a central cell-free detection zone in the center of each well of a 96-well plate.

  • Seed cells around the stoppers, then simply remove stoppers when cells have adhered to the surface to start the assay. Choose tissue culture treated, collagen I coated, or fibronectin coated plates.
  • Use Oris™ for maximal flexibility in assay design.
  • Use Oris™ if your cells require a long adhesion period.
  • Use Oris™ with the Oris™ Detection Mask for analysis on plate readers that lack virtual masking.

Oris™ Pro assays use a soluble gel to create a central cell-free detection zone in the center of each well of 96- or 384-well plates.

  • Seed cells directly in the collagen I coated wells manually or with a dispensing robot; the assay can start after the gel dissolves – no washes are needed as the gel is non-toxic.
  • Use Oris™ Pro for simplest assay setup and full automation.
  • Use Oris™ Pro for High-Throughput Screening (HTS).
  • Use Oris™ Pro for lowest cost per data point.
  • Use Oris™ Pro for highest quality optics.

Comparison of Cell Migration Assays

cell migration assays 

Cell Invasion Assays

Cell Invasion assays allow you to monitor cell movement through an extracellular matrix, in three (3) dimensions.  Cell invasion is of particular concern in cancer research, where invading tumor cells lead to metastasis, the deadliest aspect of the disease.

Platypus Technologies’ Cell Invasion Assays allow you to monitor real-time cellular behavior in a physiological relevant micro-environment that more closely resembles in vivo conditions.

Advantages:

3D Embedded Invasion Assay Detection Zone

  • 3D Embedded Invasion mimics in vivo conditions
  • Easy to use
  • Real-time monitoring
  • Quantification with plate readers, microscopes or high-content imagers
  • No artificial membranes

Oris™ Cell Invasion Assay

Assays use the same physical stopper barriers as Oris™ Migration assays, and allow you to create a consistent 3-dimensional exclusion zone in the center of each well.

  • Seed cells in collagen I solution around the stoppers. When collagen is polymerized, remove stoppers and fill center with fresh collagen I.

Oris™ Pro Cell Invasion Assay

Assays use the same soluble gel as Oris™ Pro Migration assays and provide a collagen I overlay solution into which invasive cells may penetrate.

  • Seed cells directly in the collagen I coated wells manually or with a dispensing robot; the gel dissolves as cell adhere – no washes are needed as the gel is non-toxic. Overlay with collagen I to complete assay setup.

 

Oris™ is a trademark of Platypus Technologies, LLC. Oris™ technology is protected by US patents 7,842,499, 8,268,614 and 8,512,974 and pending applications. Oris™ Pro technology is patent pending.