Oris™ 3D Cell Invasion Assay

Convenient Setup, Physiologically Relevant Results

In the Oris™ 3D Invasion Assay, stoppers are used to create a central collagen-containing Detection Zone for cell invasion experiments.

Cells are suspended in a thick layer of collagen surrounding a circular collagen Detection Zone.  After incubation, invading cells move into the detection zone, where they can be unambiguously quantified using a microscope or a plate-reader.

Above: Image analysis of the cells embedded in collagen confirm 3D distribution of cells.

Features & Benefits

  • Monitor cell invasion entirely in 3D
  • Oris™ platform enables you to distinguish different cell behavior in 2D migration and 3D invasion assays
  • Physiologically Relevant: No artificial membranes or inserts required
  • Versatile: Supports real-time monitoring or end-point analysis
  • Easy Analysis: Invasion can be quantified using image analysis or plate readers; no elaborate cell tracking software needed

Literature

Check out our poster at the ASCB Conference HERE.

Find Protocol HERE.

Examples of Studies that used Oris™ 3D Invasion Assay:

  • X. Zhang et al. “Actin cytoskeleton reorganization of hepatic stellate” Cell Adh Migr, 2019, LINK.
  • L. Bertier et al. “Downregulation of invadopodium formation” Biomed. Pharm. 2018, LINK
  • P. Masuzzo et al. “Analysis of high-throughput single-cell migration data” Sci. Reps. 2017, LINK

Ordering Information

Product NumberProduct DescriptionPrice
EIA1

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Oris™ 3D Invasion Assay, 1-pack
Oris™ Collagen I Coated, 96-well plate with Oris™ Cell Seeding Stoppers, 1
Oris™ Detection Mask, 1
Oris™ Stopper Tool, 1
Oris™ Collagen I (Rat tail) 5 mg/mL, 2 x 2 mL
$359
EIA3

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Oris™ 3D Invasion Assay, 3-pack
Oris™ Collagen I Coated, 96-well plate with Oris™ Cell Seeding Stoppers, 3
Oris™ Detection Mask, 1
Oris™ Stopper Tool, 1
Oris™ Collagen I (Rat tail) 5 mg/mL, 6 x 2 mL
$995
EIAST

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Oris™ 3D Invasion Assay, starter pack, for 48 wells
Oris™-compatible 96-well Collagen I Coated plate, 1
Oris™ Cell Seeding Stoppers, 2 x 24
Oris™ Detection Mask, 1
Oris™ Stopper Tool, 1
Oris™ Collagen I (Rat tail) 5mg/mL, 1 x 2mL
$255
CI48

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Oris™ Collagen I (Rat tail) 5 mg/mL$78

Oris™ is a trademark of Platypus Technologies, LLC. Oris™ technology is protected by US patents 7,842,499, 8,268,614 and 8,512,974 and pending applications.