Cell Invasion Assay | Collagen | 96 wells | Oris

$499.00

3D Assay for Investigating Embedded Cell Movement through Type I Collagen

  • Plate: 96-well plate with inserted Oris stoppers
    • Quantity: 1 plate, 96-wells per plate, treated with type I collagen
  • Ancillary: Oris detection mask; Oris stopper removal tool
  • Cellular matrix: Type I Collagen, 5 mg/mL
    • Quantity: 2 vials, 2 mL/vial
  • Packaging: Plate is vaccum packaged inside metal-lined pouch with humidity absorber.  Collagen vials are packaged in ice.

Protocol & Instructions  |  Frequently Asked Questions (FAQs)  |  Poster

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Description

Oris™ : A SIMPLE PLATFORM FOR PUBLICATION-READY DATA

In the Oris™ 3D Cell Invasion Assay, stoppers are used to create a central collagen-containing Detection Zone for cell invasion experiments.

Cells are suspended in a thick layer of collagen surrounding a circular collagen Detection Zone.  After incubation, invading cells move into the detection zone, where they can be unambiguously quantified using a microscope or a plate-reader.

Features & Benefits

  • Monitor cell invasion entirely in 3D
  • Oris™ platform enables you to distinguish different cell behavior in 2D cell migration and 3D cell invasion assay
  • Physiologically Relevant: No artificial membranes or inserts required
  • Versatile: Supports real-time monitoring or end-point analysis
  • Easy Analysis: Invasion can be quantified using image analysis or plate readers; no elaborate cell tracking software needed

Applications

Cell invasion assays are important tools for cancer research.  Scientists studying cancer perform cell invasion assays to measure the cell movement through an extracellular matrix. By varying the conditions of the cell culture (e.g. adding new drugs), scientists can identify conditions that prevent or accelerate cell invasion.  Through these studies, new treatments for cancer can be identified for potential therapeutic use.

Examples of Studies that used Oris™ 3D Invasion Assay:

  • J. Gu et al. “Targeting radiation-tolerant cells […]” Neuro-Oncology, 2022, LINK
  • X. Zhang et al. “Actin cytoskeleton reorganization of hepatic stellate” Cell Adh Migr, 2019LINK.
  • L. Bertier et al. “Downregulation of invadopodium formation” Biomed. Pharm. 2018LINK
  • P. Masuzzo et al. “Analysis of high-throughput single-cell migration data” Sci. Reps. 2017LINK

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