Oris™ Cell Migration Assay

Oris™ exclusion zone technology ensures consistent cell-free regions for accurate, reproducible quantification of cell movement across the plate surface. Choose Tissue Culture (TC) Treated, Collagen I Coated, or Fibronectin Coated plates:

Pre-migration and Post-migration slide

  • Industry-standard 96-well format
  • Real-time monitoring
  • Quantification with plate readers, microscopes, or high-content imagers
  • No artificial membranes
  • Consistent cell-free zone size and location for best statistics

Easy to Use

Oris™ assays use physical “stopper” barriers to create a central cell-free detection zone in the center of each well of a 96-well plate. To use Oris™ assays, simply:

  1. Seed adherent cells around the stoppers
  2. Put the plates in the incubator to promote cellular adhesion to the well bottoms
  3. Remove stoppers, add treatment, and return the plates to the incubator

There are no artificial membranes or inserts in the light path through which cells must pass. Moreover, you can monitor cell migration in real time, allowing you to acquire richer data sets and to develop assays faster. Check out this video to see how the assay is performed:


Flexible Quantification Methods

orsisCM_ProcessUse plate readers, microscopes, or high-content imagers to quantify your results:

  • With no membranes or inserts in your way, you can view cells and quantify movement at any time points during the assay
  • To use plate readers to quantify assays, stain cells then clip the patented Oris™ Detection Mask to the bottom of the plate. The mask ensures only fluorescence in the exclusion zone reaches the detector.

Robust Data

Scratch assays are cheap, but Oris™ (left) demonstrates more consistent results with greater reproducibility compared to results obtained using the typical scratch assay (right):

Oris™ Cell Migration Assay Chart - Compairison to competition

The standard Oris™ assay protocol was followed with 30,000 ECFC cells per well. These slow-adhering cells were allowed to attach overnight, then stoppers were removed and culture medium containing Dasatinib to the indicated concentrations was added. Cells were incubated for 24 hours and migration was quantified by measuring the percent area closure. Percent inhibition was then calculated as [(area of cell migration in controls – area of migration in drug treated cells) / (area of cell migration in controls – area of cell migration in samples treated with maximum concentration of drug)]. Standard deviations are for averages of four data points per drug concentration for Oris™ and eight per drug for scratch. Z-factors were 0.7 for Oris™ vs 0.2 for scratch assays (see reference).

Oris™ assays generate more robust data to:

  • Enhance your ability to identify migration inhibitors and promoters
  • Reduce the number of replicates needed, thereby reducing time and expense
  • Generate the results you need sooner and more reliably

Not sure whether to order TC Treated, Collagen I Coated, or Fibronectin Coated plates?

Test the Oris™ Cell Migration Assay – TriCoated product. Each plate has 32 wells of each of the three coatings so you can test for yourself which coating works best in your experiments.

Want to coat the plate with your own matrix or need a smaller sample size?

Have greater customization of the Oris™ Cell Assay by using your own protein matrices. Use the Oris™ Cell Migration Assembly Kit with your own matrix for a custom coated assay.

Order the Oris™ Cell Migration Assays

Product Number Product Description Package Size Price
CMA1.101 Oris™ Cell Migration Assay, 1-pack:

Oris™ 96-well plate with Oris™ Cell Seeding Stoppers, 1

Oris™ Detection Mask, 1

Oris™ Stopper Tool, 1
1-pack $299
CMA5.101 Oris™ Cell Migration Assay, 5-pack:

Oris™ 96-well plates with Oris™ Cell Seeding Stoppers, 5

Oris™ Detection Mask, 1

Oris™ Stopper Tool, 1
5-pack $1,345
CMACC1.101 Oris™ Cell Migration Assay - Collagen I Coated, 1-pack:

Oris™ Collagen I Coated, 96-well plate with Oris™ Cell Seeding Stoppers, 1

Oris™ Detection Mask, 1

Oris™ Stopper Tool, 1
1-pack $319
CMACC5.101 Oris™ Cell Migration Assay - Collagen I Coated, 5-pack:

Oris™ Collagen I Coated, 96-well plates with Oris™ Cell Seeding Stoppers, 5

Oris™ Detection Mask, 1

Oris™ Stopper Tool, 1
5-pack $1,430
CMAFN1.101 Oris™ Cell Migration Assay - Fibronectin Coated, 1-pack:

Oris™ Fibronectin Coated, 96-well plate with Oris™ Cell Seeding Stoppers, 1

Oris™ Detection Mask, 1

Oris™ Stopper Tool, 1
1-pack $339
CMAFN5.101 Oris™ Cell Migration Assay - Fibronectin Coated, 5-pack:

Oris™ Fibronectin Coated, 96-well plates with Oris™ Cell Seeding Stoppers, 5

Oris™ Detection Mask, 1

Oris™ Stopper Tool, 1
5-pack $1,490
CMATR1.101 Oris™ Cell Migration Assay - TriCoated, 1-pack:

Oris™ TriCoated (Tissue Culture Treated, Collagen I Coated, Fibronectin Coated), 96-well plate with Oris™ Cell Seeding Stoppers, 1

Oris™ Detection Mask, 1

Oris™ Stopper Tool, 1
1-pack $349
CMATR5.101 Oris™ Cell Migration Assay - TriCoated, 5-pack:

Oris™ TriCoated (Tissue Culture Treated, Collagen I Coated, Fibronectin Coated), 96-well plates with Oris™ Cell Seeding Stoppers, 5

Oris™ Detection Mask, 1

Oris™ Stopper Tool, 1
5-pack $1,500
CMA.IRT Oris™ Stopper Tool 1 $30
CMA.MM Oris™ Detection Mask 5 $50

Oris™ is a trademark of Platypus Technologies, LLC. Oris™ technology is protected by US patents 7,842,499, 8,268,614 and 8,512,974 and pending applications. Oris™ is a trademark of Platypus Technologies, LLC
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