How do I quantify Oris™ or Oris™ Pro Assays?
Oris™ and Oris™ Pro assays are designed to work with any plate reader, microscope, or high content imager that is compatible with the industry-standard microplate format. Several application notes detail the use of Oris™ with plate readers and high content imagers. The table below summarizes your options:
| Instrument | Oris™ | Oris™ Pro & Pro 384 | Comments |
|---|---|---|---|
| Standard Plate Reader | X | Requires fluorescent staining and Oris™ physical mask. Plate reader should have the detector below the plate | |
| Plate Reader with Virtual Masking Software | X | X | Requires fluorescent staining. Check with vendor that software can mask the 2 mm central circular detection zone |
| Microscope, Inverted | X | X | Automated motor-driven stage and camera recommended for efficient analysis |
| High Content Imager | X | X | |
| Platypus Bill | The Platypus bill can sense many things but alas can’t quantify Oris™ assays |
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