There are several options, simplest first:
- Run the assay for less time than the cell doubling time to limit proliferation.
- Add an inhibitor of proliferation such as mitomycin C or actinomycin D that does not inhibit migration or invasion
- Immunostain with an anti-Ki67 antibody, a marker found only on proliferating cells, and discount Ki67-positive cells from the results.
- Monitor the assay by video microscopy so each cell’s history during the assay. This a time-consuming and data-heavy approach requiring expensive equipment.
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