How can I distinguish cell migration from proliferation in Oris assays?

There are several options, simplest first:

• Run the assay for less time than the cell doubling time to limit proliferation.
• Add an inhibitor of proliferation such as mitomycin C or actinomycin D that does not inhibit migration or invasion
• Immunostain with an anti-Ki67 antibody, a marker found only on proliferating cells, and discount Ki67-positive cells from the results.
• Monitor the assay by video microscopy so each cell’s history during the assay. This a time-consuming and data-heavy approach requiring expensive equipment.