(96-well w/ Stoppers)
|Oris™ Pro Plates
(96-well w/ BCG)
|Oris™ Pro 384 Plates
(384-well w/ BCG)
|Diameter of Well – Bottom||6.3 mm||6.58 mm||3.3 mm|
|Diameter of Well – Top||6.45 mm||6.96 mm||3.7 mm|
|Diameter of Cell Free Detection Zone||2 mm||2 mm||2 mm|
|Well Volume||400 µL||392 μL||131 µL|
|Suggested culture medium per Well||100 µL||100 µL||20 µL|
|Area of Outer Annular Region (cell seeding region)||28.03 mm2||30.86 mm2||6.86 mm2|
|Area of Central Detection Zone||3.14 mm2||3.14 mm2||3.14 mm2|
|Plate Height||14.85 mm||14.4 mm||14.4 mm|
|Plate Height with Lid||17.9 mm||17 mm||16.5 mm|
|Offset of Wells (A-1 location, X)||14.32 mm||14.38 mm||12.13 mm|
|Offset of Wells (A-1 location, Y)||11.25 mm||11.24 mm||8.99 mm|
|Distance between Wells||9.0 mm||9.0 mm||4.5 mm|
|Well Depth||12.1 mm||10.9 mm||11.5 mm|
|Thickness of Well Bottom||0.25 mm||190 μm ±10%||190 μm ±10%|
|Storage||4°C||15 – 30°C||15 – 30°C|
If your imaging system is provided with a software package, your vendor can help you with Oris™ quantification, and may already have a routine specifically designed for Oris™. See this application note for an example.
Some plate readers have limited clearance and may not accept the plate with the lid on.
The mask provided with the Oris™ assays has an aperture diameter of 2.1 mm while the size of the Detection Zone formed by the stopper is 2.0 mm. The larger mask aperture is designed to allow some fluorescent background signal to included in the light path in order to bring the detector into the appropriate dynamic range, as illustrated in this technical note.