Archives: FAQ

What are the dimensions of Oris™ and Oris™ Pro plates?

Oris™ Plates
(96-well w/ Stoppers)
Oris™ Pro Plates
(96-well w/ BCG)
Oris™ Pro 384 Plates
(384-well w/ BCG)
Diameter of Well – Bottom 6.3 mm 6.58 mm 3.3 mm
Diameter of Well – Top 6.45 mm 6.96 mm 3.7 mm
Diameter of Cell Free Detection Zone 2 mm 2 mm 2 mm
Well Volume 400 µL 392 μL 131 µL
Suggested culture medium per Well 100 µL 100 µL 20 µL
Area of Outer Annular Region (cell seeding region) 28.03 mm2 30.86 mm2 6.86 mm2
Area of Central Detection Zone 3.14 mm2 3.14 mm2 3.14 mm2
Plate Height 14.85 mm 14.4 mm 14.4 mm
Plate Height with Lid 17.9 mm 17 mm 16.5 mm
Offset of Wells (A-1 location, X) 14.32 mm 14.38 mm 12.13 mm
Offset of Wells (A-1 location, Y) 11.25 mm 11.24 mm 8.99 mm
Distance between Wells 9.0 mm 9.0 mm 4.5 mm
Well Depth 12.1 mm 10.9 mm 11.5 mm
Thickness of Well Bottom 0.25 mm 190 μm ±10% 190 μm ±10%
Storage 4°C 15 – 30°C 15 – 30°C

What software can I use to quantify cell migration/invasion in images I capture from Oris™ Assays?

We recommend ImageJ software, or its close relative Fiji for users who don’t have a commercial software package provided with the imaging system to be used. Both are freeware.

If your imaging system is provided with a software package, your vendor can help you with Oris™ quantification, and may already have a routine specifically designed for Oris™. See this application note for an example.

/ In / By Joe Lampe / Comments Off on What software can I use to quantify cell migration/invasion in images I capture from Oris™ Assays?

I sometimes see “crescent effects” under the microscope with the Oris™ Detection Mask in place. Is this a problem for quantification using a microplate reader?

The mask provided with the Oris™ assays has an aperture diameter of 2.1 mm while the size of the Detection Zone formed by the stopper is 2.0 mm. The larger mask aperture is designed to allow some fluorescent background signal to included in the light path in order to bring the detector into the appropriate dynamic range, as illustrated in this technical note.

/ In / By Joe Lampe / Comments Off on I sometimes see “crescent effects” under the microscope with the Oris™ Detection Mask in place. Is this a problem for quantification using a microplate reader?