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Our poster
“High Content Analysis
of an Automatable
3-Dimensional
Cell Invasion Assay”
won the
Top Ten
Poster Award
at the SBS 2010!
The Oris™ Pro Cell Invasion Assay enables the use of automated liquid handling equipment for cell seeding and allows unlimited access to cells throughout the experiment. The Oris™ Pro Cell Invasion Assay uses a non-toxic biocompatible gel, BCG (see Figure 1: Well Bottom with BCG), to form a centrally located and temporary cell-free zone on culture surfaces. Cells are seeded into the 96-well plate and attach in a monolayer around the BCG. The BCG dissolves to reveal a Detection Zone and a Collagen I Overlay is added to create a 3-D ECM environment for cell invasion (see Figure 2: Assay Schematic). Cells can be treated with one or multiple stains or labels. The cells in the Detection Zone can be quickly captured and easily quantified in real-time using microscopes and High Content Screening (HCS)/High Content Imaging (HCI) instruments. Cell invasion above the plate surface can be assessed by performing a Z-stack analysis. Staining live cells at the beginning of the experiment permits real-time tracking of cell movement that can be captured at any time point. Both endpoint and kinetic data can be generated.
Features & Benefits of the Oris™ Pro Cell Invasion Assay
Figure 3: Oris™ Pro Collagen I Cell Invasion Analysis: HT-1080 cells were seeded onto an Oris™ Pro Collagen I plate. Following the addition of a 3.0mg/mL Collagen I gel overlay, cell invasion was permitted for 72 hours in the presence of 10% FBS. Cells were fixed and stained with TRITC-phalloidin and DAPI. Images were captured using the BD Pathway™ 855 Bioimaging System and the number of invading cells in the Detection Zone was quantified at defined Z intervals. Based on a Z-stack analysis of 8 wells, 416 cells +/- 38 invaded 60-150 um into the Collagen I Overlay in the Detection Zone. Images of pre-invasion (A) and invasion at 72 hr (B-E) are shown (phalloidin pseudo-colored yellow), in addition to images at Z-intervals of 60 um (C) and 150 um (D) into the Collagen I gel. A color-coded composite of Z-stacks at intervals of 60, 90, 120, and 150 um obtained from a single well is shown (E).
ORDER the Oris™ Pro Cell Invasion Assay
| Product Number | Product Description | Price |
|---|---|---|
| PROCIACC1 | $310 | |
| PROCIACC2 | $600 |
* Patent Pending.
Oris™ is a trademark of Platypus Technologies, LLC
BD Pathway™ 855 Bioimaging System is a registered trademark of BD Biosciences.
