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The Oris™ Pro Cell Migration Assay enables the use of automated liquid handling equipment for cell seeding and allows unrestricted access to cells throughout the experiment. The Oris™ Pro Assay uses a non-toxic biocompatible gel, BCG (see Figure 1: Well Bottom with BCG), to form a cell-free zone on culture surfaces. After seeding cells into the 96-well plate, the BCG dissolves permitting cells to migrate into the well centers (see Figure 2: Assay Schematic). Oris™ Pro Assays* are available with Tissue Culture Treated or Collagen I Coated surfaces. Cells can be stained and the cells in the Detection Zone can be captured and quantifed in real-time using microscopes and High Content Screening (HCS)/High Content Imaging (HCI) instruments. Staining live cells at the beginning of the experiment permits real-time images to be captured at any time point. Both endpoint and kinetic data can be generated.
Features & Benefits of the Oris™ Pro Cell Migration Assay
Figure 3: Oris™ Pro Cell Migration Analysis: HUVECs were seeded onto an Oris™ Pro Collagen I plate. A dose-response titration was performed using the actin polymerization inhibitor, Cytochalasin D. Cells were treated for 18 hours, fixed, and stained for F-actin. Images were acquired using the BD Pathway™ 855 Bioimaging System and a dose-response curve with IC50 was generated (n=16).
ORDER the Oris™ Pro Cell Migration Assay
| Product Number | Product Description | Price |
|---|---|---|
| PROCMA1 | Oris™ Pro Cell Migration Assay, 1-pack | $144 |
| PROCMA5 | Oris™ Pro Cell Migration Assay, 5-pack |
$672 |
| PROCMACC1 | Oris™ Pro Cell Migration Assay - Collagen I Coated, 1-pack |
$155 |
| PROCMACC5 | Oris™ Pro Cell Migration Assay - Collagen I Coated, 5-pack |
$725 |
* Patent Pending.
Oris™ is a trademark of Platypus Technologies, LLC
BD Pathway™ 855 Bioimaging System is a registered trademark of BD Biosciences.
