The Oris Pro Cell Migration Assay enables the use of automated liquid handling equipment for cell seeding and allows unrestricted access to cells throughout the experiment. The Oris Pro Assay uses a non-toxic biocompatible gel, BCG (see Figure 1: Well Bottom with BCG), to form a cell-free zone on culture surfaces. After seeding cells into the 96-well plate, the BCG dissolves permitting cells to migrate into the well centers (see Figure 2: Assay Schematic). Oris Pro Assays* are available with Tissue Culture Treated or Collagen I Coated surfaces. Cells can be stained and the cells in the Detection Zone can be captured and quantifed in real-time using microscopes and High Content Screening (HCS)/High Content Imaging (HCI) instruments. Staining live cells at the beginning of the experiment permits real-time images to be captured at any time point. Both endpoint and kinetic data can be generated.
Figure 3: Oris Pro Cell Migration Analysis: HUVECs were seeded onto an Oris Pro Collagen I plate. A dose-response titration was performed using the actin polymerization inhibitor, Cytochalasin D. Cells were treated for 18 hours, fixed, and stained for F-actin. Images were acquired using the BD Pathway 855 Bioimaging System and a dose-response curve with IC50 was generated (n=16).
|Product Number||Product Description||Price|
|PROCMA1||Oris Pro Cell Migration Assay, 1-pack||$144|
|PROCMA5||Oris Pro Cell Migration Assay, 5-pack
|PROCMACC1||Oris Pro Cell Migration Assay - Collagen I Coated, 1-pack
|PROCMACC5||Oris Pro Cell Migration Assay - Collagen I Coated, 5-pack
* Patent Pending.
Oris is a trademark of Platypus Technologies, LLC
BD Pathway 855 Bioimaging System is a registered trademark of BD Biosciences.