Place An Order:
Toll-Free: 1.866.296.4455 Fax: 608.237.1271
Get creative with your cell migration, invasion, and 2-D closure assays!
Platypus Technologies has developed a product line that gives researchers more control over designing a cell migration assay. Each Oris™ Cell Migration Assembly kit contains the Oris™ Cell Seeding Stoppers for creating a detection zone at the center of each well in a 96-well plate. Since the stoppers are not pre-inserted into the wells, researchers have two options; coat the plate with an extracellular matrix component or use the coated plate provided to design an assay. Create unique 3-dimensional overlays in each well and watch how cells invade and respond to various compounds (chemokinesis). The Oris™ Universal Cell Migration assembly Kit is an ideal alternative to the scratch assay for studying wound healing. The detection zone created by the Oris™ stopper provides a consistent area for a 2-D closure assay.
The original Oris™ Universal Cell Migration Assembly kit is supplied with a 96-well, black, clear-bottom plate, an Oris™ Detection Mask, an Oris™ Stopper Tool, and 96 Oris™ Cell Seeding Stoppers.*
The NEW Oris™ Cell Migration Assembly Kit-FLEX now allows for more control over how many tests can be performed in a single experiment. Now use anywhere from 4 to 96 test wells per experiment; it is your choice. Whether you are studying cancer metastasis or wound healing, the Oris™ Cell Migration Assembly Kit-FLEX adapts to your unique experimental design.
Oris™ Cell Seeding Stopper Insertion Video
Features & Benefits of assays designed using Oris™ Cell Migration Assembly kits
Cell Migration and Cell Invasion Assay Design Options
| Cell Migration Assay | Cell Invasion Assay |
|---|---|
|
1. Insert Oris™ Cell Seeding Stoppers 2. Seed each well with cells (to achieve 90-95% confluency around Oris™ Stoppers) 3. Incubate plate to allow cell adherance to bottom of well 4. Remove Oris™ Stoppers 5. Allow cells to migrate into the detection zone 6. Stain cells 7. Measure migration with a microplate reader, microscope or digital imaging system |
1. Coat Oris™-compatible, 96-well plate with choice of ECM (unless already coated) 2. Insert Oris™ Cell Seeding Stoppers 3. Seed each well with cells (to achieve 90-95% confluency around Oris™ Stoppers) 4. Incubate plate to allow cell adherance to bottom of well 5. Remove Oris™ Stoppers 6. Overlay adherent cells with ECM 7. Allow cells to invade into the detection zone 8. Stain cells 9. Measure invasion with a microplate reader, microscope or digital imaging system |
Figure 1: Oris™ Cell Migration Analysis - Endpoint and Kinetic Detection: Wells were seeded with 50,000 HT-1080 cells. Cells adhered after 4 hours and the Oris™ Cell Seeding Stoppers were removed. All wells received CellTracker™ Green to fluorescently stain the cells. The assay was then incubated for 28 hours to permit cell migration and at various time points the fluorescence signals in the detection zones were measured using a plate reader. The images above illustrate representative data from pre-migration (t=0 hrs) and post-migration (t = 21 hrs) wells. The graph depicts a real-time analysis of cell migration that was prepared by transposing the fluorescent signal into cell numbers.
NOTE: Manually inserting Oris™ Cell Seeding Stoppers will result in data with higher CV values than data obtained using the Oris™ Cell Migration Assay kits pre-populated with mechanically inserted stoppers.
ORDER the Oris™ Cell Migration Assembly Kits
| Product Number | Product Description | Package Size | Price |
|---|---|---|---|
| CMAUFL4 | Oris™ Cell Migration Assembly Kit - FLEX: Oris™ compatible, 96-well plates, 4 Oris™ Cell Seeding Stoppers, 4 packs of 24* Oris™ Detection Mask, 1 Oris™ Stopper Tool, 1 * sufficient materials for 96 tests |
1-kit | $325 |
| CMAU101 | Oris™ Universal Cell Migration Assembly Kit, 1-pack: Oris™-compatible, 96-well plate (black, clear bottom), 1 Oris™ Cell Seeding Stoppers, 96 Oris™ Detection Mask, 1 Oris™ Stopper Tool, 1 |
1-pack | $220 |
| CMAU505 | Oris™ Universal Cell Migration Assembly Kit, 5-pack: Oris™-compatible, 96-well plates (black, clear bottom), 5 Oris™ Cell Seeding Stoppers, 5 x 96 Oris™ Detection Mask, 1 Oris™ Stopper Tool, 1 |
5-pack | $1,040 |
| CMAUCC1 | Oris™ Cell Migration Assembly Kit - Collagen I Coated, 1-pack: Oris™ Collagen I Coated, 96-well plate, 1 Oris™ Cell Seeding Stoppers, 96 Oris™ Detection Mask, 1 Oris™ Stopper Tool, 1 |
1-pack | $230 |
| CMAUCC5 | Oris™ Cell Migration Assembly Kit - Collagen I Coated, 5-pack: Oris™ Collagen I Coated, 96-well plates, 5 Oris™ Cell Seeding Stoppers, 5 x 96 Oris™ Detection Mask, 1 Oris™ Stopper Tool, 1 |
5-pack | $1,095 |
| CMA.IRT | Oris™ Stopper Tool |
1 | $20 |
| CMA.MM | Oris™ Detection Mask |
5 | $35 |
* Patent Pending
Oris™ is a trademark of Platypus Technologies, LLC.
CellTracker™ Green is a trademark of Invitrogen Corporation.
View Platypus' Terms & Conditions.
