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Adherent cell migration without cell culture inserts
The Oris™ Cell Migration Assay* is designed with a unique cell seeding stopper, detection mask, and stopper tool. There is no Transwell® membrane insert needed. This unique plate design generates highly reproducible results using a microscope, digital imaging system or fluorescence plate reader. Simply adhere the seeded cells in each well, remove the stopper to create a detection zone, and measure the migration (see Figure 1: Assay Schematic). Oris™ assays are available with tissue culture treated plates, Collagen I coated plates, Fibronectin coated plates, or a TriCoated version that contains all 3 surfaces. Wound healing assays are normally performed using a scratch assay, which has high variability. The Oris™ Cell Migration Assay is an ideal alternative to the scratch assay since the cell seeding stoppers create a consistent detection zone to monitor 2-D closure.
Oris™ Cell Migration Assay Video
Features & Benefits of the Oris™ Cell Migration Assay
Click HERE to view electronic versions of Platypus posters presented at the recent ASCB and AACR meetings.
Figure 2: Oris™ Cell Migration Analysis - Endpoint and Kinetic Detection: Wells were seeded with 50,000 HT-1080 cells. Cells adhered after 4 hours and the Oris™ stoppers were removed. All wells received CellTracker™ Green to fluorescently stain the cells. The assay was then incubated for 28 hours to permit cell migration and at various time points the fluorescence signals in the detection zones were measured using a plate reader. The images above illustrate representative data from pre-migration (t=0 hrs) and post-migration (t = 21 hrs) wells. The graph depicts a real-time analysis of cell migration that was prepared by transposing the fluorescent signal into cell numbers.
Figure 3: Oris™ Cell Migration Analysis - Inhibition: 3T3-Swiss albino fibroblast cells were pretreated with 5 ug/mL MMC to inhibit proliferation, and then 25,000 cells/well were seeded and allowed to adhere. Stoppers were then removed, Latrunculin A (0 - 1000 nM) was added to the wells, and the plate was incubated to permit cell migration. The cells were labeled with Calcein AM and the fluorescence quantified in the detection zone using a Synergy Bio-Tek plate reader. Latrunculin A inhibition of 3T3-Swiss albino cell migration was demonstrated by using the Oris™ Cell Migration Assay. The inhibitory effect of Latrunculin A was dose-dependent and complete inhibition of 3T3-Swiss albino cell migration was detected in the presence of at least 500 nM Latrunculin A.
ORDER the Oris™ Cell Migration Assays
| Product Number | Product Description | Package Size | Price |
|---|---|---|---|
| CMA1.101 | Oris™ Cell Migration Assay, 1-pack: Oris™ 96-well plate with Oris™ Cell Seeding Stoppers, 1 Oris™ Detection Mask, 1 Oris™ Stopper Tool, 1 |
1-pack | $220 |
| CMA5.101 | Oris™ Cell Migration Assay, 5-pack: Oris™ 96-well plates with Oris™ Cell Seeding Stoppers, 5 Oris™ Detection Mask, 1 Oris™ Stopper Tool, 1 |
5-pack | $1,040 |
| CMACC1.101 | Oris™ Cell Migration Assay - Collagen I Coated, 1-pack: Oris™ Collagen I Coated, 96-well plate with Oris™ Cell Seeding Stoppers, 1 Oris™ Detection Mask, 1 Oris™ Stopper Tool, 1 |
1-pack | $230 |
| CMACC5.101 | Oris™ Cell Migration Assay - Collagen I Coated, 5-pack: Oris™ Collagen I Coated, 96-well plates with Oris™ Cell Seeding Stoppers, 5 Oris™ Detection Mask, 1 Oris™ Stopper Tool, 1 |
5-pack | $1,095 |
| CMAFN1.101 | Oris™ Cell Migration Assay - Fibronectin Coated, 1-pack: Oris™ Fibronectin Coated, 96-well plate with Oris™ Cell Seeding Stoppers, 1 Oris™ Detection Mask, 1 Oris™ Stopper Tool, 1 |
1-pack | $240 |
| CMAFN5.101 | Oris™ Cell Migration Assay - Fibronectin Coated, 5-pack: Oris™ Fibronectin Coated, 96-well plates with Oris™ Cell Seeding Stoppers, 5 Oris™ Detection Mask, 1 Oris™ Stopper Tool, 1 |
5-pack | $1,125 |
| CMATR1.101 | Oris™ Cell Migration Assay - TriCoated, 1-pack: Oris™ TriCoated (Tissue Culture Treated, Collagen I Coated, Fibronectin Coated), 96-well plate with Oris™ Cell Seeding Stoppers, 1 Oris™ Detection Mask, 1 Oris™ Stopper Tool, 1 |
1-pack | $330 |
| CMATR5.101 | Oris™ Cell Migration Assay - TriCoated, 5-pack: Oris™ TriCoated (Tissue Culture Treated, Collagen I Coated, Fibronectin Coated), 96-well plates with Oris™ Cell Seeding Stoppers, 5 Oris™ Detection Mask, 1 Oris™ Stopper Tool, 1 |
5-pack | $1,515 |
| CMA.IRT | Oris™ Stopper Tool |
1 | $20 |
| CMA.MM | Oris™ Detection Mask |
5 | $35 |
* Patent Pending.
Oris™ is a trademark of Platypus Technologies, LLC
CellTracker™ Green is a trademark of Invitrogen Corporation.
Transwell® is a registered trademark of Corning, Inc.
