Oris™ & Oris™ Pro Cell Migration & Invasion Assays



Oris™ exclusion zone technology ensures consistent cell-free regions for accurate, reproducible quantification of cell movements, whether migration, invasion or wound closure:

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  • Industry-standard 96- or 384-well formats
  • Real-time monitoring
  • Quantification with plate readers, microscopes or high-content imagers
  • No artificial membranes
  • Use Oris™ for maximal assay flexibility
  • Use Oris™ Pro for full automation with high Z factors

Oris™ assays use physical “stopper” barriers to create a central cell-free detection zone in the center of each well of a 96-well plate.

  • Use Oris™ for maximal flexibility in assay design.
  • Use Oris™ if your cells require a long adhesion period.
  • Use Oris™ with the Oris™ Detection Mask for analysis on plate readers that lack virtual masking.

Oris™ Pro assays use a soluble gel to create a central cell-free detection zone in the center of each well of 96- or 384-well plates.

  • Use Oris™ Pro for simplest assay setup and full automation.
  • Use Oris™ Pro for lowest cost per data point.
  • Use Oris™ Pro for highest quality optics.

Migration assays

Migration assays monitor cell movement in 2 dimensions across a surface:

Oris™ Migration assays use physical “stopper” barriers to create a central cell-free detection zone in the center of each well of a 96-well plate.

  • Seed cells around the stoppers, then simply remove stoppers when cells have adhered to the surface to start the assay. Choose tissue culture treated, collagen I coated, or fibronectin coated plates.

Oris™ Pro Migration assays use a soluble gel to create a central cell-free detection zone in the center of each well of 96- or 384-well plates.

  • Seed cells directly in the collagen I coated wells manually or with a dispensing robot; the assay can start after the gel dissolves – no washes are needed as the gel is non-toxic.

Invasion assays

Invasion assays monitor cell movement in 3 dimensions through an extracellular matrix:

Oris™ 3D Embedded Invasion assays use the same physical stopper barriers as Oris™ Migration assays, and allow you to create a consistent 3-dimensional exclusion zone in the center of each well.

  • Seed cells in collagen I solution around the stoppers. When collagen is polymerized, remove stoppers and fill center with fresh collagen I.

Oris™ Pro Invasion assays use the same soluble gel as Oris™ Pro Migration assays and provide a collagen I overlay solution into which invasive cells may penetrate.

  • Seed cells directly in the collagen I coated wells manually or with a dispensing robot; the gel dissolves as cell adhere – no washes are needed as the gel is non-toxic. Overlay with collagen I to complete assay setup.

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Oris™ is a trademark of Platypus Technologies, LLC. Oris™ technology is protected by US patents 7,842,499, 8,268,614 and 8,512,974 and pending applications. Oris™ Pro technology is patent pending.